apc anti ulbp4 (R&D Systems)

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R&D Systems apc anti ulbp4
Apc Anti Ulbp4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ulbp4/Human+ULBP-4%2FRAET1E+APC-conjugated+Antibody/pm40510363-81-21-22
Average 93 stars, based on 5 article reviews

apc anti ulbp4 - by Bioz Stars, 2026-09

93/100 stars

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Staining:

Article Title: Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells
Article Snippet: For cytofluorimetric analysis, cells were stained with the following fluorochrome-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-anti-CD105 (clone MEM-226, IgG2a; Immunotools, 21271054); fluorescein isothiocyanate (FITC)-anti-CD3 (clone UCHT1, IgG1; 300406), FITC-antiCD47 (clone CC2C6, IgG1; 323106), PE-anti-CD73 (clone AD2, IgG1; 344004), PE-anti-NKp30 (clone P30-15, IgG1; 325208) purchased from Biolegend; PE-anti-CD107a (clone H4A3, IgG1; 121079942), PE-anti-CD146 (clone P1H12, IgG1; 12146942) from eBioscience; allophycocyanin (APC)anti-CD56 (clone NCAM16.2, IgG2b; 341027), PE-anti-PDL1 (clone MIH1, IgG1; 557924), PE-anti-PD-L2 (clone MIH18, IgG1; 558066) from Becton Dickinson; PE-antiNKp46 (clone BAB281, IgG; IM3711), PE-anti-NKp44 (clone Z231, IgG1; IM3710), PE-anti-NKG2A (clone Z199, IgG2b; IM329IU), PE-anti-NKG2D (clone ON72, IgG1; A08934), PEanti-2B4 (clone C1.7, IgG1; IM1608) from Beckman Coulter; APC-anti-CD90 (clone DG3, IgG1; 130095402); PE-anti-HLA class I (clone Tu149, IgG2a; MHBC04) from Invitrogen. .. For indirect staining, the following primary monoclonal antibodies (mAbs) were used: anti-ULBP1 (clone 170818, IgG2a; MAB 1380100), anti-ULBP2 (clone 165903, IgG2a; MAB 1298100), anti-ULBP3 (clone 166510, IgG2a; MAB 1517100), anti-ULBP4 (clone 709116, IgG2b; MAB 6285), antiB7-H6 mAb (clone 875001, IgG1; MAB7144), anti-Nidogen-1 (clone 302117, IgG1; MAB2570) all from R&D Systems; The following mAbs were produced in our laboratory: F22 (IgG1, anti-DNAM-1); 5B14 (IgM, anti-B7-H3), L14 (anti-Nectin-2, IgG2a), L95 (anti-PVR, IgG1), BAM195 (anti-MICA, IgG1), A6136 (anti-HLA class I, IgM). .. The goat anti-mouse antibodies PE-anti-IgG1 (Life Technologies; P21129), PE-anti-IgG2a (Southern Biotech; 108009), PE-anti-IgG2b (Southern Biotech; 109009), PE-antiIgM (Beckman Coulter; IM0555), and PE AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, 109–116-170) were used as secondary reagents.

Article Title: Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells
Article Snippet: For cytofluorimetric analysis, cells were stained with the following fluorochrome-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-anti-CD105 (clone MEM-226, IgG2a; Immunotools, 21271054); fluorescein isothiocyanate (FITC)-anti-CD3 (clone UCHT1, IgG1; 300406), FITC-anti-CD47 (clone CC2C6, IgG1; 323106), PE-anti-CD73 (clone AD2, IgG1; 344004), PE-anti-NKp30 (clone P30-15, IgG1; 325208) purchased from Biolegend; PE-anti-CD107a (clone H4A3, IgG1; 121079942), PE-anti-CD146 (clone P1H12, IgG1; 12146942) from eBioscience; allophycocyanin (APC)-anti-CD56 (clone NCAM16.2, IgG2b; 341027), PE-anti-PD-L1 (clone MIH1, IgG1; 557924), PE-anti-PD-L2 (clone MIH18, IgG1; 558066) from Becton Dickinson; PE-anti-NKp46 (clone BAB281, IgG; IM3711), PE-anti-NKp44 (clone Z231, IgG1; IM3710), PE-anti-NKG2A (clone Z199, IgG2b; IM329IU), PE-anti-NKG2D (clone ON72, IgG1; A08934), PE-anti-2B4 (clone C1.7, IgG1; IM1608) from Beckman Coulter; APC-anti-CD90 (clone DG3, IgG1; 130095402); PE-anti-HLA class I (clone Tu149, IgG2a; MHBC04) from Invitrogen. .. For indirect staining, the following primary monoclonal antibodies (mAbs) were used: anti-ULBP1 (clone 170818, IgG2a; MAB 1380100), anti-ULBP2 (clone 165903, IgG2a; MAB 1298100), anti-ULBP3 (clone 166510, IgG2a; MAB 1517100), anti-ULBP4 (clone 709116, IgG2b; MAB 6285), anti-B7-H6 mAb (clone 875001, IgG1; MAB7144), anti-Nidogen-1 (clone 302117, IgG1; MAB2570) all from R&D Systems; The following mAbs were produced in our laboratory: F22 (IgG1, anti-DNAM-1); 5B14 (IgM, anti-B7-H3), L14 (anti-Nectin-2, IgG2a), L95 (anti-PVR, IgG1), BAM195 (anti-MICA, IgG1), A6136 (anti-HLA class I, IgM). .. The goat anti-mouse antibodies PE-anti-IgG1 (Life Technologies; P21129), PE-anti-IgG2a (Southern Biotech; 108009), PE-anti-IgG2b (Southern Biotech; 109009), PE-anti-IgM (Beckman Coulter; IM0555), and PE AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, 109–116-170) were used as secondary reagents.

Bioprocessing:

Article Title: Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells
Article Snippet: For cytofluorimetric analysis, cells were stained with the following fluorochrome-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-anti-CD105 (clone MEM-226, IgG2a; Immunotools, 21271054); fluorescein isothiocyanate (FITC)-anti-CD3 (clone UCHT1, IgG1; 300406), FITC-anti-CD47 (clone CC2C6, IgG1; 323106), PE-anti-CD73 (clone AD2, IgG1; 344004), PE-anti-NKp30 (clone P30-15, IgG1; 325208) purchased from Biolegend; PE-anti-CD107a (clone H4A3, IgG1; 121079942), PE-anti-CD146 (clone P1H12, IgG1; 12146942) from eBioscience; allophycocyanin (APC)-anti-CD56 (clone NCAM16.2, IgG2b; 341027), PE-anti-PD-L1 (clone MIH1, IgG1; 557924), PE-anti-PD-L2 (clone MIH18, IgG1; 558066) from Becton Dickinson; PE-anti-NKp46 (clone BAB281, IgG; IM3711), PE-anti-NKp44 (clone Z231, IgG1; IM3710), PE-anti-NKG2A (clone Z199, IgG2b; IM329IU), PE-anti-NKG2D (clone ON72, IgG1; A08934), PE-anti-2B4 (clone C1.7, IgG1; IM1608) from Beckman Coulter; APC-anti-CD90 (clone DG3, IgG1; 130095402); PE-anti-HLA class I (clone Tu149, IgG2a; MHBC04) from Invitrogen. .. For indirect staining, the following primary monoclonal antibodies (mAbs) were used: anti-ULBP1 (clone 170818, IgG2a; MAB 1380100), anti-ULBP2 (clone 165903, IgG2a; MAB 1298100), anti-ULBP3 (clone 166510, IgG2a; MAB 1517100), anti-ULBP4 (clone 709116, IgG2b; MAB 6285), anti-B7-H6 mAb (clone 875001, IgG1; MAB7144), anti-Nidogen-1 (clone 302117, IgG1; MAB2570) all from R&D Systems; The following mAbs were produced in our laboratory: F22 (IgG1, anti-DNAM-1); 5B14 (IgM, anti-B7-H3), L14 (anti-Nectin-2, IgG2a), L95 (anti-PVR, IgG1), BAM195 (anti-MICA, IgG1), A6136 (anti-HLA class I, IgM). .. The goat anti-mouse antibodies PE-anti-IgG1 (Life Technologies; P21129), PE-anti-IgG2a (Southern Biotech; 108009), PE-anti-IgG2b (Southern Biotech; 109009), PE-anti-IgM (Beckman Coulter; IM0555), and PE AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, 109–116-170) were used as secondary reagents.

Produced:

Article Title: Stromal-like Wilms tumor cells induce human Natural Killer cell degranulation and display immunomodulatory properties towards NK cells
Article Snippet: For cytofluorimetric analysis, cells were stained with the following fluorochrome-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-anti-CD105 (clone MEM-226, IgG2a; Immunotools, 21271054); fluorescein isothiocyanate (FITC)-anti-CD3 (clone UCHT1, IgG1; 300406), FITC-anti-CD47 (clone CC2C6, IgG1; 323106), PE-anti-CD73 (clone AD2, IgG1; 344004), PE-anti-NKp30 (clone P30-15, IgG1; 325208) purchased from Biolegend; PE-anti-CD107a (clone H4A3, IgG1; 121079942), PE-anti-CD146 (clone P1H12, IgG1; 12146942) from eBioscience; allophycocyanin (APC)-anti-CD56 (clone NCAM16.2, IgG2b; 341027), PE-anti-PD-L1 (clone MIH1, IgG1; 557924), PE-anti-PD-L2 (clone MIH18, IgG1; 558066) from Becton Dickinson; PE-anti-NKp46 (clone BAB281, IgG; IM3711), PE-anti-NKp44 (clone Z231, IgG1; IM3710), PE-anti-NKG2A (clone Z199, IgG2b; IM329IU), PE-anti-NKG2D (clone ON72, IgG1; A08934), PE-anti-2B4 (clone C1.7, IgG1; IM1608) from Beckman Coulter; APC-anti-CD90 (clone DG3, IgG1; 130095402); PE-anti-HLA class I (clone Tu149, IgG2a; MHBC04) from Invitrogen. .. For indirect staining, the following primary monoclonal antibodies (mAbs) were used: anti-ULBP1 (clone 170818, IgG2a; MAB 1380100), anti-ULBP2 (clone 165903, IgG2a; MAB 1298100), anti-ULBP3 (clone 166510, IgG2a; MAB 1517100), anti-ULBP4 (clone 709116, IgG2b; MAB 6285), anti-B7-H6 mAb (clone 875001, IgG1; MAB7144), anti-Nidogen-1 (clone 302117, IgG1; MAB2570) all from R&D Systems; The following mAbs were produced in our laboratory: F22 (IgG1, anti-DNAM-1); 5B14 (IgM, anti-B7-H3), L14 (anti-Nectin-2, IgG2a), L95 (anti-PVR, IgG1), BAM195 (anti-MICA, IgG1), A6136 (anti-HLA class I, IgM). .. The goat anti-mouse antibodies PE-anti-IgG1 (Life Technologies; P21129), PE-anti-IgG2a (Southern Biotech; 108009), PE-anti-IgG2b (Southern Biotech; 109009), PE-anti-IgM (Beckman Coulter; IM0555), and PE AffiniPure F(ab’)2 Fragment Goat Anti-Human IgG (Jackson ImmunoResearch, 109–116-170) were used as secondary reagents.

Incubation:

Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), anti- ULBP1 (1:50; MAB1380, R&D Systems), anti-ULBP3 (1:50; MAB1517, R&D Systems), anti-ULBP4 (1:50; D ow nloaded from http://ahajournals.org by on January 12, 2024 MAB6285, R&D Systems), anti-NeuN (1:250; ab177487, abcam), anti-GFAP (1:1000; 13-0300, Invitrogen), anti- CX3CR1 (1:100; ab8021, abcam) and anti-CD68 (1:100; PA5-32330, Invitrogen). ..

Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), antiULBP1 (1:50; MAB1380, R&D Systems), anti-ULBP3 (1:50; MAB1517, R&D Systems), anti-ULBP4 (1:50; MAB6285, R&D Systems), anti-NeuN (1:250; ab177487, abcam), anti-GFAP (1:1000; 13-0300, Invitrogen), antiCX3CR1 (1:100; ab8021, abcam) and anti-CD68 (1:100; PA5-32330, Invitrogen). ..

Binding Assay:

Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), antiULBP1 (1:50; MAB1380, R&D Systems), anti-ULBP3 (1:50; MAB1517, R&D Systems), anti-ULBP4 (1:50; MAB6285, R&D Systems), anti-NeuN (1:250; ab177487, abcam), anti-GFAP (1:1000; 13-0300, Invitrogen), antiCX3CR1 (1:100; ab8021, abcam) and anti-CD68 (1:100; PA5-32330, Invitrogen). ..

Review